Article

Projet
Molécules ciblant les conformations spécifiques des acides nucléiques  
Martine DEMEUNYNCKIsabelle BAUSSANNE,   
Titre
Selective Luminescent Labeling of DNA and RNA Quadruplexes by p-Extended Ruthenium Light-Up Probes  
[Full paper ]
Auteurs
D. Saadallah, M. Bellakhal, S. Amor, J.-F. Lefebvre, M. Chavarot-Kerlidou, I. Baussanne, C. Moucheron, M. Demeunynck, D. Monchaud
Edition
Chem. Eur. J. 2017, 23, 4967–4972
Année
2017
Résumé
A series of Ru II complexes exhibiting p-extended, acridine-based ancillary chelating heterocycles display high affinity and selectivity for DNA and RNA quadruplexes. The most promising candidates (3, 4) possess remarkable light-up luminophore properties (up to 330-fold luminescence enhancement upon interaction with quadruplexes), enabling them to discriminate quadruplexes from genomic DNA owing to a photochemical mechanism involving DNA protection against non-radiative decay (DAND), thus deviating from the other complexes of this series of ligands that exhibit an excited-state intramolecular proton transfer (ESIPT) that quenches their luminescence. The in vitro and preliminary in cellulo results shown here confirm the interest of this new family of fluorophores as invaluable molecular tools to detect G-quadruplexes in cells.